Poly(adenosine diphosphate ribose) polymerase in Physarum polycephalum

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Poly(adenosine diphosphate ribose) polymerase in Physarum polycephalum nuclei.

1. The isolated nuclei of the slime mould Physarum polycephalum contain an enzyme that will incorporated [adenine-3H] NAD+ into an acid-insoluble product, which is shown to be poly(ADP-ribose). 2. This incorporation has an optimum pH of 8.2 and a temperature optimum below 10degreesC. 3. Optimum stimulation is given by 15 mM-Mg2+. 4. 2-Mercaptoethanol or dithiothreitol also stimulates the incorp...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1975

ISSN: 0264-6021

DOI: 10.1042/bj1470119